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Extraction Add 150 ul 0.1% TFA, 60% ACN, 60’ rocking at RT Remove supernatant to a siliconized 0.5 ml eppendorf-style tube Re-extract the slices with 150 ul 0.1% TFA, 60% ACN as above Combine supernatants Reduction and Alkylation / Digestion / Extraction / Print
Extraction
Add 150 ul 0.1% TFA, 60% ACN, 60’ rocking at RT
Remove supernatant to a siliconized 0.5 ml eppendorf-style tube
Re-extract the slices with 150 ul 0.1% TFA, 60% ACN as above
Combine supernatants
Reduction and Alkylation / Digestion / Extraction / Print
Analytical Services:
Amino Acid Analysis
Protein Sequence Analysis
Chromatography
Electrophoresis
Mass Spectrometry
Protocols:
SDS PAGE
Staining
Electroblotting
In Gel Digestion
PVDF Membrane Digestion
Fee Schedules:
Academic
Commercial
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Analytical Services:
Protocols:
Fee Schedules: