Digestion Buffers
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50 mM Tris pH 9.2, 1% Reduced Triton X-100, 10% Acetonitrile (for
EKC)
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100 mM ammonium bicarbonate, 1% Reduced Triton X-100,10% Acetonitrile (for
trypsin)
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Reducing Solution: Add 10 ml (1M stock) DTT to 0.99 ml PCS2
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Alkylating Solution: Add 1.42 gm acrylamide to 8.2 ml PCS2 (Hewlett Packard)
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PCS2: Guanidine-HCL, pH 8.3
Buffers / Reduction
& Alkylation / Digestion / Extraction
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