Extraction Spin sample briefly to collect liquid; sonicate 5 min at 30°C Remove supernatant fluids with gel loading tip to siliconized test tube Add 50 ml of digestion buffer, vortex and sonicate 5 min at 30°C Remove supernatant fluids and pool with first supernatant materials Add 100 ml of 0.1% TFA to the membranes; vortex and sonicate 5 min at 30°C Remove supernatant fluids and pools with first supernatant materials Buffers / Reduction & Alkylation / Digestion / Extraction / Print

Analytical Services: Amino Acid Analysis Protein Sequence Analysis Chromatography Electrophoresis Mass Spectrometry   Protocols: SDS PAGE Staining Electroblotting In Gel Digestion PVDF Membrane Digestion   Fee Schedules: Academic Commercial

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Last Edited: February 06, 2004