Reduction and Alkylation Wet membrane with 100%MeOH Soak membrane in 100 ml Reducing Solution at 50C for 10 minutes Place membrane in 100 ml Alkylating Solution at 50C for 5 minutes Rinse membrane 1 X with 250 ml 100 mM Tris pH 9.2 Rinse membrane in 500 ml Digestion buffer Buffers / Reduction & Alkylation / Digestion / Extraction / Print

Analytical Services: Amino Acid Analysis Protein Sequence Analysis Chromatography Electrophoresis Mass Spectrometry   Protocols: SDS PAGE Staining Electroblotting In Gel Digestion PVDF Membrane Digestion   Fee Schedules: Academic Commercial

Home / Instrumentation / References / Forms / Contact / News / Archive / Links

Calabreso Web Designs
please e-mail all errors to the webmaster
Last Edited: February 06, 2004