Electrotransfer of Proteins to PVDF Membrane for Sequence
Analysis
This protocol is preferred for proteins destined
to automated Edman sequencing and is amenable to all other
downstream cleavage methods. The
use of CAPS buffer is important in order to eliminate the high
concentrations of glycine that result from Towbin buffer that will
interfere with the ability to accurate determine the protein
sequence of the protein. The
method can be used for electroblotting for Western analysis, but is
more costly due to the higher cost of the CAPS.
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