Required Solutions
Electroblot Buffer:
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10 mM CAPS
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10% methanol
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pH 11.0
10x Concentrated Stock:
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Measure ~900 ml MilliQ water
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Add 22.13 gm CAPS
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Titrate to pH 11.0 with concentrated NaOH
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Bring to 1000 ml with additional water.
1X Working Solution:
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800 ml Milli-Q water
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Add 100 ml HPLC grade MeOH
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Add 100 ml 10x concentrated stock of CAPS
Required
Solutions / Procedure / Alternative
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