Alternative Stains
Coomassie blue R-250: 0.1% Coomassie blue in 50%
methanol/1% acetic acid, 5 min. Destain: 1-3 times with 50%
methanol/1% acetic acid (5 min) followed by several washes with
MilliQ water.
Coomassie blue G-250: Stain for one to several minutes.
Destain with water. (More sensitive than Amido Black, but
‘dirtier’. Causes higher sequencer background noise).
Ponceau S: 0.1% Ponceau S in 1% acetic acid, 5 min.
Destain: 1% acetic acid, followed by several MilliQ water washes.
(Least sensitive but very clean stain. Low sequencer noise)
Required
Solutions / Procedure / Alternative
Stains / Print
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