Step One
Assemble the glass plate sandwich of the
electrophoresis apparatus according to manufacturer’s instructions
by stacking, in order, the notched aluminum plate, two 0.75 mm
spacers, and the larger rectangular glass plate. Be sure to align
the spacers properly with the ends flush with the top and bottom
edge of the two plates when positioning the sandwiches. The gel(s)
can be assembled and locked into the gel casting stand using the
clamps and eccentric cams. In either case, it is critical to align
the spacers properly to prevent leaks during gel pouring.
We often will place a piece of Parafilm, folded in
half, beneath the gel sandwich, to help form a tight seal.
Additionally, a small dab of Vaseline can be placed at the corner of
the gel near the spacers to help prevent leaks due to uneveness of
the spacers.
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