Step Two Prepare the appropriate concentration separating gel solution as needed using the accompanying table as a guideline. Preparing 25 ml of solution is sufficient for two mini gels with 0.75 mm spacers. Any excess gel solution can be used as a monitor of the polymerization process. Add ammonium persulfate and TEMED last.   1 - 2 - 3 - 4 - 5 - 6 - 7 - 8 - 9 - 10 - 11 - Print

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