Step Three Immediately after adding the ammonium persulfate and TEMED, pipet the solution into the gel plate sandwich to the appropriate height. This can be determined by measuring using a sample comb prior to loading the gel solution. Remember to leave enough room to accommodate a stacking gel that is ~1cm high.   1 - 2 - 3 - 4 - 5 - 6 - 7 - 8 - 9 - 10 - 11 - Print

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