Pouring SDS Polyacrylamide Gels

Step Five

After the gel is polymerized, remove the iso-butanol or water and rinse the top of the gel with 1 x Tris HCl/SDS pH8.8. This can be left in place overnight if appropriate. Otherwise, rinse the surface of the gel with MilliQ water and pour a stacking gel.
Polymerized gels can be stored for up to one week at 4°C wrapped in SaranWrap.

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Analytical Services:

Amino Acid Analysis

Protein Sequence Analysis

Chromatography

Electrophoresis

Mass Spectrometry

Protocols:

SDS PAGE

Staining

Electroblotting

In Gel Digestion

PVDF Membrane Digestion

Fee Schedules:

Academic

Commercial

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Last Edited: February 06, 2004