Pouring SDS Polyacrylamide Gels

To pour the stacking gel, remove any overlay buffer (or water) and partially insert the Teflon sample comb in the gel sandwich at an angle. Prepare the stacking gel solution (5 ml is enough for two gels) and pipet into the space above the separating gel so that it begins to fill in around the "teeth" of the sample comb. Push the comb down into the gel solution in a way so that no air bubbles are trapped. Add additional acrylamide solution as necessary to fill the spaces in the comb completely. Allow the gel to polymerize (~30 minutes).