Step Eight
Turn the power off and load the samples using a Hamilton syringe or gel loading tips.
We recommend that samples destined for sequencing NOT be boiled but rather heated in sample buffer to 50-60°C for 15 minutes. This is usually sufficient for total denaturation.
Preparing samples at approximately the same concentration and loading an equal volume to each well will ensure that all lanes are the same width and that the proteins run evenly. If unequal volumes of sample buffer are added to the wells, the lane with the larger volume will spread during electrophoresis and constrict adjacent lanes, causing distortions.
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- 11 - Print
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