Destaining Silver Stained Gel Slices (for
Blum Silver Stained Gels)
Solutions needed: (prepare fresh)
30 mM potassium ferricyanide; 98 mg/10 ml water
100 mM sodium thiosulfate; 248 mg/10 ml water
Procedure:
Mix solutions together 1:1 (this is working solution)
Pipet 50 ul of working solution onto gel slice(s)
Incubate until slice is destained (several minutes)
Stop with water; discard
Cover with 250 ul of 100mM ammonium bicarb, 20 min; discard
Required Solutions
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