Non-fixing Silver Staining (Blum's) Fixative: Acetic acid 10% (v/v), ethanol 30% (v/v) Measure 100 ml Glacial Acetic Acid Measure 300 ml ethanol To 1000 ml with MilliQ water Rinse: 20% (v/v) ethanol Sensitizer: 0.02% (w/v) sodium thiosulfate Weigh 0.2 gm sodium thiosulfate Dissolve in 1000 ml MilliQ water Silver nitrate: 0.2% (w/v) silver nitrate Measure 2.0 gm silver nitrate Dissolve in 1000 ml MilliQ water Developer: 0.3% (w/v) sodium carbonate 0.025% (v/v) formaldehyde Sodium thiosulfate 10 mgm/L Measure 15 gm sodium carbonate Measure 25 ml of stock sodium thiosulfate Measure 125 ul 37% formaldehyde To 500 ml with MilliQ water Stop: Tris, acetic acid Measure 50 gm Tris base Measure 25 ml Glacial acetic acid To 1000 ml with MilliQ water   Required Solutions / Procedure / Destaining / Print

Analytical Services: Amino Acid Analysis Protein Sequence Analysis Chromatography Electrophoresis Mass Spectrometry   Protocols: SDS PAGE Staining Electroblotting In Gel Digestion PVDF Membrane Digestion   Fee Schedules: Academic Commercial

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