Protein Microsequencing
Gas- or Pulsed Liquid-Phase Sequence Analysis. Geisow, M.J. and Aitken, A. (1989) in Protein Sequencing A Practical Approach (Findlay, J.B. C. and Geisow, M.J. eds.) pp. 85-98, IRL Press, New York, N.Y.
Matsudaira, P. (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidinedifluoride membranes. J. Biol. Chem. 262: 10035-10038.
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SDS Polyacrylamide Gel Electrophoresis
Schagger, H. and Von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379.
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680-685.
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2D Gel Electrophoresis
O'Farrell, P.H., (1975), "High resolution
Two-Dimensional Electrophoresis of Proteins," J. Biol. Chem. 250,
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Electroblotting
Moos, M. et al. (1988). Reproducible high-yield sequencing of proteins electrophoretically and transferred to an inert support. J. Biol. Chem. 263: 6005-6008.
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Mozdzanowsky, J., Hembach, P. and Speicher, D.W. (1992). High yield electroblotting onto polyvinylidinedifluoride membranes from polyacrylamide gels. Electrophoresis 13: 59-64.
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Patterson, S.D., Hess, D., Yungwirth, T., and Aebersold, R. (1992). High-yield recovery of electroblotted proteins and cleavage fragments from a cationic polyvinylidene fluoride-based membrane. Anal. Biochem. 202, 193-203.
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Lauriere, M. (1993). A semidry electroblotting system efficiently transfers both high- and low-molecular-weight proteins separated by SDS-PAGE. Anal. Biochem. 212, 206-211.
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Shultz, J., Improved staining of small PVDF-immobilized proteins. Promega Notes 36.
Gentile, F., Bali, E. and Pignalosa, G. (1997). Sensitivity and applications of the nondenaturing staining of proteins on polyvinylidenedifluoride membranes with amido black 10B in water followed by destaining in water. Anal. Biochem. 245, 260-262.
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Silver Staining
Rabilloud, Mechanisms of protein silver staining in
polyacrylamide gels: A 10-year synthesis. Elctrophoresis, 1990, 11,
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Rabilloud, A comparison between low background
silver diamine and silver nitrate protein stains, Ecltrophoresis,
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Rabilloud et al, Silver-staining of proteins in
polyacrylamide gels: a general overview. Cellular and Molecular
Biology, 1994, 40, 57-75. [PubMed
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Yan et al, A modified silver staining protocol for
visualization of proteins compatible with matrix-assisted laser
desorption/ionization and electrospray ionization-mass spectrometry.
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Sinha et al, A new silver staining apparatus and
procedure for matrix-assisted laser desorption/ionization-time of
flight analysis of proteins after two-dimensional electrophoresis,
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Gharahdaghi et al, Mass spectromectric
identification of proteins from silver-stained polyacrylamide gel: a
method for the removal of silver ions to enhance sensitivity.
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Sumner et al, Silver stain removal using H2O2
for enchanced peptide mass mapping by matrix-assisted laser
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In Gel Digestion Procedures
Hellman, U. et al. (1995). Improvement of an "In-Gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing. Anal. Biochem. 224, 451-455.
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Jeno, P. et al. (1995). Internal sequences from proteins digested in polyacrylamide gels. Anal. Biochem.
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Stone, K.L. and Williams, K.R. (1993) In A Practical Guide to Protein and Peptide Purification for Microsequencing, 2nd ed. (P. Matsuidaira, ed.) pp. 43-69. Academic Press, San Diego.
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