Instrumentation
Automated Edman chemistry is performed on a Hewlett Packard
G1000A Automated Protein Sequencer. The G1000A is a complete,
automated chemistry and analytical system for amino acid sequence
analysis of protein and peptide samples in picomole to nanomole
amounts. The system is composed of the G1000A Protein Sequencer, an
on-line PTH-amino acid analyzer based on the HP 1090M HPLC and a
system controller for coordinated, single-point control.
The G1000A Protein Sequencer performs automated Edman sequencing
chemistry on a miniature, biphasic, C18 resin-packed column sample
support. The reagent delivery system includes a bi-directional flow
path, facilitating the design of chemical methods that exploit the
surface properties of the column packing material and the polarities
of the reagents, solvents, and sample.
Instrumentation /
Sample Submission /
Sample Preparation / Sequencing Methods /
Results / Data Evaluation
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Form