Protein Sequence Analysis

Samples will be subjected to Edman chemistry by the appropriate method as dictated by the way in which the sample was presented to the Protein Chemistry Laboratory (Method 3.1 for liquid protein samples; Method 3.5 for liquid peptide samples; Method PVDF 3.1 for electroblotted protein samples). The details of all current methods are available in our laboratory.

All samples are preceded by PTH amino acid standards for calibration purposes. The ternary HPLC system and chromatographic method separate the 19 standard PTH-amino acids, sequencing by-products and several modified forms of PTH-cysteine to greater than 98% resolution. Retention times of the standards are used for peak identification. Customized reports are generated using the HP Chemstation software giving chromatograms of each cycle, repetitive yield graphs and histograms. A blank HPLC trace is included in all experiments to evaluate background signal. Blank runs are included in the raw data along with your sample.