Sequencing Results
After each cycle of Edman chemistry, the PTH-amino acid is
analyzed by the on-line amino acid analyzer. Each cycle of sequence
is presented as a chromatogram of the PTH amino acid. Sequences were
called by comparing amounts of amino acids present in each cycle. In
cases where more than one amino acid were seen in a cycle, the amino
acid whose amount most closely corresponded with the rest of the
sequence (based on amount and operator judgement) was called.
Residues that have reduced yields (serine and threonine) were taken
into account.
Cycles where no amino acid was seen are denoted with
"X". It should be noted that if your sample was not
reduced and alkylated, a cycle designated by X might actually
contain a cysteine residue. Alternatively, some other residue that
does not appear on the PTH-amino acid standard may be present (phosphoamino
acid, glycosylated amino acid, lipid-modified). Cycles where the
sequence ended (the end of the peptide/protein were reached) are so
denoted.
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